Analytical Characterization &QC Workshop
10/6/2020 8:21:28 AM
SPR characterization of antibody binding kinetic to FcγRIIIa variants as indicator of the antibody potency: impact of galactose and fucose of a human IgG1 antibody
In the field of therapeutic mAbs, especially for those who mediate ADCC (Antibody Dependent Cellular Cytotoxicity), the attention is focus on their Fc-domain glycosylation.
In the present study, SPR methods were developed to characterize the binding kinetic of differently glycosylated human IgG1 to FcγRIIIa variants, which are the receptors mediating ADCC.
An expected significant impact of core-fucose on binding kinetic to FcγRIIIa was observed, confirming the diagnostic capability of the methods. Furthermore, a strong correlation between Fc galactosylation, binding affinity for FcγRIIIa variants and ADCC was found, demonstrating the potential of SPR-based method to predict the antibody potency during the process development for antibody manufacturing.
In the photo: Dr. Mariani (speaker)
09 October (Japan) - Click here to join